Journal: Metabolites

Article Title: Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.)

doi: 10.3390/metabo13050598

Figure Lengend Snippet: Chemical composition and antioxidant activity of cloudberry fruits and leaves’ water–ethanol extracts (mg g −1 ).

Article Snippet: An amperometric determination of the antioxidant activity (AOA) was carried out on a Blizar AOA analyzer (Interlab, Moscow, Russia) consisting of a chromatographic pump, a loop injector, and an electrochemical detector with a glassy carbon working electrode [ ].

Techniques: Antioxidant Activity Assay

Journal: Metabolites

Article Title: Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( Rubus chamaemorus L.)

doi: 10.3390/metabo13050598

Figure Lengend Snippet: Fractions of the cloudberry leaf dichloromethane–methanol extract and their general characteristics.

Article Snippet: An amperometric determination of the antioxidant activity (AOA) was carried out on a Blizar AOA analyzer (Interlab, Moscow, Russia) consisting of a chromatographic pump, a loop injector, and an electrochemical detector with a glassy carbon working electrode [ ].

Techniques: Antioxidant Activity Assay

Journal: The EMBO Journal

Article Title: Pathogenic fungi neutralize plant‐derived ROS via Srpk1 deacetylation

doi: 10.15252/embj.2022112634

Figure Lengend Snippet: Volcano plot displaying the differentially regulated genes (fold change > 2, FDR < 0.05) in the K304Q strain compared with the WT. Both of the strains were pre‐treated with 10 mM H 2 O 2 for 1 h. RNA‐seq analysis of six downregulated antioxidant enzymes. Differential expression in three biological replicates illustrated using a heat map with colored squares indicating the range of expression referred to as the FPKM value. qRT‐PCR validation of antioxidant enzymes in the indicated strains under oxidative stress. The expression levels were normalized to that of the Fol actin gene. Data are presented as mean ± SD. The presence of different letters above the mean values of three replicates indicates a significant difference between different samples ( P < 0.05, ANOVA).

Article Snippet: Some antioxidant enzymes can be secreted by plant pathogens (Tanabe et al , ; Huang et al , ).

Techniques: RNA Sequencing, Quantitative Proteomics, Expressing, Quantitative RT-PCR, Biomarker Discovery

Journal: The EMBO Journal

Article Title: Pathogenic fungi neutralize plant‐derived ROS via Srpk1 deacetylation

doi: 10.15252/embj.2022112634

Figure Lengend Snippet: Relative expression level of six antioxidant enzymes during the infection stage. The experiments were carried out as shown in Fig . Data are presented as mean ± SD. The presence of different letters above the mean values of three biological replicates indicates a significant difference between different samples ( P < 0.05, ANOVA). Secretion of the FOXG_17180‐GFP, FOXG_15294‐GFP, and FOXG_13788‐GFP proteins. The proteins extracted from mycelia (M) and culture supernatants (S) were analyzed by Western blotting with anti‐GFP and anti‐Actin. Each gel shown is a representative experiment carried out three times. The loading controls were Fol Actin and CBB staining. Determination of the H 2 O 2 content in tomato roots after infection by the indicted strains at 0–5 dpi. The bars denote the standard errors of three replicates. Pathogenicity of the indicated strains in tomato 14 days after infection. Quantification of the disease indexes of the indicated strains in D (four biological replicates). qRT‐PCR analysis of Fol EF‐1α transcript levels in tomato roots 14 days after infection by the indicated strains. The expression of tomato RCE1 was used as a control to ensure the use of equal amounts of RNA for RT‐PCR. Data information: For E and F, the presence of different letters above the mean values of four replicates indicates a significant difference between different samples ( P < 0.05, ANOVA). The whiskers of the boxplots indicate the upper and lower quartiles, the boxes indicate the interquartile range, and the plus sign indicates the mean. Source data are available online for this figure.

Article Snippet: Some antioxidant enzymes can be secreted by plant pathogens (Tanabe et al , ; Huang et al , ).

Techniques: Expressing, Infection, Western Blot, Staining, Quantitative RT-PCR, Control, Reverse Transcription Polymerase Chain Reaction

Journal: The EMBO Journal

Article Title: Pathogenic fungi neutralize plant‐derived ROS via Srpk1 deacetylation

doi: 10.15252/embj.2022112634

Figure Lengend Snippet: A Phosphorylation of FolSr1 and FolSr1 Δ112–114 Δ147–149 in the WT and ΔFolSrpk1 strains. Eluted proteins bound to the anti‐GFP beads were analyzed by the anti‐Ser/Phosphoserine and anti‐GFP antibodies (Abcam). The amount of phosphorylated and total FolSr1 in the WT was each set as 1. B Phosphorylation of FolSr2 in the WT and ΔFolSrpk1 strains. C Subcellular localization of FolSr1‐GFP. Arrows indicate the nucleus. Scale bars = 5 μm. D–F Co‐IP (D), Y2H (E), and BiFC (F) assays showing the interaction of FolSrpk1 with FolSr1. Arrows indicate the nucleus. Scale bars = 5 μm. G Sequence alignments of FolSr1 with its orthologs from F. graminearum (FGRRES_09864) and S. pombe (SPBC11C11.08). The two RS regions were marked with lines. Arrows indicate the four conserved phosphorylation sites S112, S114, S147, and S149. H qRT‐PCR analysis of antioxidant‐related genes in the ΔFolSr1:FolSr1 and ΔFolSr1:FolSr1 Δ112–114 Δ147–149 strains. Data are presented as mean ± SD of three biological replicates (** P < 0.05 by unpaired two‐tailed t ‐test). I Phosphorylation of FolSr1‐GFP during the infection process. Total proteins were extracted from tomato roots at 0 (conidia) and 1–5 dpi. Eluted proteins bound to the anti‐GFP beads were analyzed by the anti‐Ser/Phosphoserine and anti‐GFP antibodies. The amount of phosphorylated and total FolSr1 at 0 dpi was each set as 1. Data information: For A, B, D, and I, each gel shown is a representative experiment carried out three times. Source data are available online for this figure.

Article Snippet: Some antioxidant enzymes can be secreted by plant pathogens (Tanabe et al , ; Huang et al , ).

Techniques: Phospho-proteomics, Co-Immunoprecipitation Assay, Sequencing, Quantitative RT-PCR, Two Tailed Test, Infection

Journal: Antioxidants

Article Title: The Antioxidant Mechanism of Peptides Extracted from Tuna Protein Revealed Using a Molecular Docking Simulation

doi: 10.3390/antiox13020166

Figure Lengend Snippet: Active peptide antioxidant activity and toxicity prediction.

Article Snippet: Antioxidant activity prediction was used for biological activity predictions over 0.5 ( https://services.healthtech.dtu.dk/services/AnOxPePred-1.0/ (accessed on 25 March 2023)).

Techniques: Antioxidant Activity Assay, Sequencing

Journal: Antioxidants

Article Title: The Antioxidant Mechanism of Peptides Extracted from Tuna Protein Revealed Using a Molecular Docking Simulation

doi: 10.3390/antiox13020166

Figure Lengend Snippet: ( A ) Frequency of different amino acids at the N–terminal and C–terminal, and ( B ) antioxidant active peptide molecular quantity distribution.

Article Snippet: Antioxidant activity prediction was used for biological activity predictions over 0.5 ( https://services.healthtech.dtu.dk/services/AnOxPePred-1.0/ (accessed on 25 March 2023)).

Techniques:

Journal: Beilstein Journal of Organic Chemistry

Article Title: A new analog of dihydroxybenzoic acid from Saccharopolyspora sp. KR21-0001

doi: 10.3762/bjoc.20.44

Figure Lengend Snippet: NMR spectroscopic data of KR21-0001A ( 1 ) in CD 3 OD.

Article Snippet: The antioxidant activity of KR21-0001A ( 1 ) was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Antioxidant Assay Kit, DOJINDO © laboratory, Japan) according to the manufacturer’s protocol.

Techniques: